发布时间 : 星期日 文章pBI121质粒图谱及载体信息更新完毕开始阅读6e1bdb1514791711cc791709
pBI121质粒图谱及载体信息
启动子：35S
终止子：Nos
% t+ f! b8 G% s报告基因：GUS
抗性基因：NptII
pBI121载体信息：
LOCUS AY781296 8196 bp DNA linear SYN 10-NOV-2004DEFINITION Expression vector pBI121_pro T-DNA region, complete sequence. ACCESSION AY781296
VERSION AY781296.1 GI:55418080 KEYWORDS .
1 R+ Y\; D$ h$ R9 @\- O, V2 p5 P: h7 c2 s+ G/ X, _2 X: K2 P% M4 G- w# R
SOURCE Expression vector pBI121_pro ORGANISM Expression vector pBI121_pro
other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8196)
AUTHORS Zeng,Y.H., Cao,J.W., Cai,H.Y., Chen,X.H. and Miao,L.X.
TITLE Construction of the plant expression vector of the proBA gene from Bacillus subtilis mutant to proline analog and analysis of the salt tolerance of transgenic Arabidopsis thaliana JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8196)
AUTHORS Zeng,Y.H., Cao,J.W., Cai,H.Y., Chen,X.H. and Miao,L.X. TITLE Direct Submission
JOURNAL Submitted (14-OCT-2004) College of Life Science, Wuhan University, Bayi Road, Wuhan, Hubei 430072, P.R.China FEATURES Location/Qualifiers source 1..8196
/organism=\ /mol_type=\ /db_xref=\ misc_feature 1..306
/note=\ gene 318..1112 /gene=\ CDS 318..1112 /gene=\ /codon_start=1 /transl_table=11
/product=\ /protein_id=\V51261.1\ /db_xref=\
/translation=\AQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF\ terminator 1113..1365 /note=\ promoter 2059..2859
/note=\ misc_feature 2872..3174
9 x5 Y0 z( o; l5 P7 z3 f! V4 Y- v t\3 x- N' }$ ?. d4 t7 T& g G2 v* b5 \\ ?& q x* X9 i1 J6 v* r* I! u7 _$ W$ O/ U6 D) a/ V\S% u+ T, T- }# b2 \\/ L0 a J6 v: M* W& P; t5 K3 ?! C% }7 U: Q3 X+ N5 \\& K, \\+ _/ _2 p# M\6 X$ d9 v\z/ ^! _8 \\ P6 l' c& o' o3 h; E1 C q9 z3 H& q- u3 Z- [- M4 B1 C8 l5 A8 B4 A/ K- d0 |4 a' u. n6 M9 y7 E$ L4 Z$ A\; R1 \\\`) D; F* l- B: I$ j+ D8 a4 F0 m' f$ ~; C- U3 * S7 s /note=\ Accession Number X86778\ gene 3175..4287 /gene=\ CDS 3175..4287 /gene=\ /codon_start=1 /transl_table=11
/product=\ /protein_id=\V51262.1\ /db_xref=\
/translation=\V
ILITSGAVAAGFSALGYPARPVTIKGKQAAAAVGQTLLMQQYMDHLKRYGLTPAQILL
TRNDFSKRERYRNAYATVMELLERGVVPIINENDSTSVEELTFGDNDMLSALVSGLIH
ADQLMILTDINGLYDANPNENPDAKRFDYLTDITPELLGYAGSAGSKVGTGGMKSKFL
AAQTALSLGVKVFIGTGAGREKLKVILDGKGDGTYIGDKELSTVNNTRQWIMFHSPIS
GEIIIDGGAEQAMIHNGSSLLPAGVADVCGSFPKGAVVEVRGPGGIIGKGQTHYSSDE
ILEAKGKRSDQLPGAKQVEVIHRNDWVNLFDEGENK\ misc_feature 4300..4602
/note=\ Accession Number X86778\ gene 4603..5733 /gene=\ CDS 4603..5733 /gene=\ /codon_start=1 /transl_table=11
/product=\ /protein_id=\V51263.1\ /db_xref=\
/translation=\ERQLI
LTENQKDIEAGQKRGLTPDIIDRLTLDEKRLLDIADAVELLIGLEDPVGESLETIQKE
NGLSIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSV
MKRALGLSKLPIDAVQLIEDTSKETAKQLFTLNDGLDVLIPRGGKNLIDLVVRESTVP
G! |2 ~: i/ r4 d) |3 L& q' F4 O1 V6 |, b, i+ Y: s/ |( z, v) S: r) F: Q\~$ S; |; O5 P+ {9 Q+ X) o- z, a6 e9 H3 h+ t& j* [( L5 l% I7 P\) X T+ p' V: }) i$ U, A# f( c4 } e7 P1 M- A6 {& f5 q& p* @% U0 [4 J\\& q: ~/ M* c5 K7 Y3 N2 j/ I& o\' Y9 Y# H7 {; W0 ^7 h; Y8 B. d% ?, a n* ~% U2 v# _( t6 ^\! j2 K# _3 o' x% N0 X( n! Q; _! M# \\& ~; J' Y( I9 q# ^5 D: @: l& C8 {1 P0 I( u: i# O6 E& z0 m9 ]& i! j7 R0 F( \\+ m# C VLETGAGNCHVYIDESADPDMARDVVINAKTQRPSVCNAIESLLIHEKWAEVHGRKLL
NQLTEKGVELRGDQMICELEPNAKQAEEADWGAEYLAPILSVKTVHDVQEAVRHIQQY
GTNHSEAILTENGEHAAYFQTAVDAAAVYHNASTRFTDGFEF\ gene 5758..7569 /gene=\ CDS 5758..7569 /gene=\ /codon_start=1 /transl_table=11
/product=\ /protein_id=\V51264.1\ /db_xref=\
/translation=\SRA
IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ\ terminator 7630..7882 /note=\ misc_feature 7883..8196
/note=\ ORIGIN
1 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta 61 agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact 121 gacagaaccg caacgattga aggagccact cagccgcggg tttctggagt ttaatgagct 181 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
\\2 g& W5 z8 W+ Y* C' }/ W# x! Y2 m- o6 t( Y5 W0 K* ^8 F H8 T3 {) j8 P' e4 ?( s8 l6 `4 g4 M* f0 o4 i: # \\* v1 5 g. & H7 f, P9 y* f8 h6 q9 c/ P/ R% l ^6 k3 S\S: 3 o. f! y A\8 g: kJ3 ], X! |3 F4 x; n\ U\. T0 Y- u4 l# l! c% ^% W& c( G, p+ }% [6 Z* A& r. |; d- q2 q7 m6 T3 s) y. P- {, K1 z5 ]7 _) V2 X' c: H7 D1 t6 ]& l' j9 w }! _; Q$ s, \\0 w9 V& L6 `3 a' [1 I& ~